Protein Biotechnology
Syllabus, Master's level, 1KB761
- Code
- 1KB761
- Education cycle
- Second cycle
- Main field(s) of study and in-depth level
- Chemistry A1F, Molecular Biotechnology A1F, Technology A1F
- Grading system
- Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
- Finalised by
- The Faculty Board of Science and Technology, 18 April 2013
- Responsible department
- Department of Chemistry - Ångström
Entry requirements
Methods in Biotechnology 5 credits or Genome Biology 5 credits or equivalent
Learning outcomes
The aim of this course is advanced knowledge about protein production on different scales, especially large-scale production, in the biotechnology industry.
After completing this course the student should be able to:
- Describe from start to finish the entire process of of protein production on different scales, including large-scale industrial production, that fulfils the quality requirements from both industry and regulations from governmental agencies (GLP,GMP).
- Use a bioreactor in different ways to produce proteins.
- Perform of protein purification with regard to molecular properties and demands placed on product purity.
- Design purification methods for protiens with respect to starting material and production scale.
- Master and rationally select different methods for characterisation of proteins, with consideration to product requirements. .
- Critically examine large scale industrial protein production processesand present the results orally. .
Content
Cell culturing bioreactors. Preparative scale protein purification and analysis . Protein characterisation by modern bioanalytical tools. GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice). Laws and regulations.Examination of large scale industrial protein production processes. Site visit at a biotechnology company.
Instruction
Instructions are in the form of lectures, seminars, laboratory practicals and a group assignment with oral presentations. Communication training within selected profile areas is integrated with the course components.
Assessment
A written test at the end of the course, and/or during the course, corresponding to 5 credits. Laboratory practicals and demonstrations, oral and written presentations of laboratory reports, and oral presentationof the group assignment t correspond to 5 credits in total. The final grade represents a weighted total of these components.
Other directives
Cannot be counted towards the degree together with 1MB572 Protein biotechnology I and 1MB573 Protein biotechnology II.