Enzymology and Bioorganic Catalysis

15 credits

Syllabus, Master's level, 1KB424

A revised version of the syllabus is available.

Code: 1KB424
Education cycle: Second cycle
Main field(s) of study and in-depth level: Chemistry A1F
Grading system: Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
Finalised by: The Faculty Board of Science and Technology, 9 March 2017
Responsible department: Department of Chemistry - BMC

Entry requirements

120 credits. 60 credits in chemistry including at least 10 credits at Master's level. Biochemistry, 15 credits.

Learning outcomes

The student should after completed course be able to

analyse structure/function relationships in biocatalysed reactions
predict possible catalytic mechanisms of given reaction types
present strategies for the analysis of kinetic mechanisms of catalysed reactions
account for industrial applications of biocatalysis

Content

Reaction kinetics of simple and complex reactions (rate equations, reaction order, molecularity, rate limiting step). Thermodynamic aspects of reactions (reaction coordinates, activated complexes and transition states). Enzyme kinetics (steadystate kinetics, presteadystate kinetics). Reaction mechanisms (ligand binding; catalytic groups: acid/base, nucleophiles, electrophiles, cofactors, metals and entropic effects). Experimental analysis of catalytic and kinetic mechanisms (spectrometry, Xray crystallography, stopped flow, isotope effects, structure/reactivity relationships). Mathematical data analysis (regression analysis, model discrimination). Modelling of enzymatic reactions. Stereo chemistry (mechanisms). Structure/function relationships (protein folding, mutagenesis, enzyme inhibition, evolution of catalytic mechanism, catalytic antibodies). Catalytic nucleic acids (ribozymes, catalytic DNA).

Instruction

The course includes lectures, groups discussions, tutorials and laboratory practicals. Participation in discussions, tutorials and laboratory practicals is mandatory. In addition, a mandatory literature project should be carried out.

Assessment

Written exam (8 credits). Mandatory course components, tutorials, laboratory practicals and literature project which is presented orally and in writing corresponds to 7 points. The final grade is weighted from the results on the written exam and the practical part.

Other directives

The course can not be included in higher education qualification together with 1KB463 Enzymology and Bioorganic Catalysis, 15 cr.