Protein Biotechnology

10 credits

Syllabus, Master's level, 1KB762

Code
1KB762
Education cycle
Second cycle
Main field(s) of study and in-depth level
Applied Biotechnology A1F, Chemistry A1F
Grading system
Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
Finalised by
The Faculty Board of Science and Technology, 30 August 2018
Responsible department
Department of Chemistry - Ångström

Entry requirements

Participation in Structure and Function of Macromolecules, 15 credits or the equivalent. Proficiency in English equivalent to the Swedish upper secondary course English 6.

Learning outcomes

The aim of this course is advanced knowledge about large-scale protein production in biotechnology industry.

On completion of the course, the student should be able to:

  • Describe the process from start to end, of large scale industrial protein production that fulfils the quality requirements from both industry and regulations from governmental agencies (GLP,GMP), including culturing, purification and analyses of proteins.
  • Use a bioreactor in different ways to produce proteins.
  • Perform different types of protein purification
  • Perform characterisation of proteins using various analytical tools.
  • Plan large scale industrial protein production processes, and organise and communicate these efficiently to a group.

Content

Cell culturing in bioreactors. Protein purification in preparative scale. Protein characterisation by modern bioanalytical tools. GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice). Laws and regulations. Group project: Planning of large scale industrial protein production processes. Site visit.

Instruction

Instructions are in the form of lectures, seminars, laboratory practicals and demonstrations, and a group project with a written report and presentations.

Assessment

A written test at the end of the course, and/or during the course, corresponding to 5 credits. Laboratory practicals and demonstrations, oral and written presentations of the laboratory reports, and oral and written report of the group planning project together corresponds to 5 credits.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

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