Entry requirements

180 credits in biology, bio-/medicine, biotechnology, pharmaceutical biosciences, medical science or equivalent education giving relevant knowledge in cell biology, genetics biochemistry and molecular biology. Proficiency in English equivalent to the Swedish upper secondary course English 6.

Learning outcomes

The course aims to provide an in-depth knowledge of molecular detection methods and their use in research and medicine.

Knowledge and understanding

On completing the course, the student shall demonstrate the ability to:

• demonstrate basic knowledge of proteins
• account for and evaluate current methods and techniques within the research field, their practical execution and application in research and diagnostics

Competence and skills

On completing the course, the student shall demonstrate the ability to:

• independently analyse, process and formulate relevant scientific questions in the research field, apply appropriate molecular methods and discuss these orally as well as in writing
• compile, critically analyse, and assess relevant scientific literature within the field of proteome analysis and diagnostics

Judgement and approach

On completing the course, the student shall demonstrate the ability to:

• compile, critically analyse and evaluate research results and present these both orally and in writing

Content

The course will provide an insight into the applications, strategies and significance of molecular analysis methods in research and medicine. Current opinions and research findings, as well as development of novel methods and technologies, will be conveyed and discussed throughout the course. Different methods for protein analysis, including a variety of affinity-based protein detection methods, will be covered. Molecular tools and technologies such as e.g. affibodies, their use in cancer therapy and LigandTracer, showing protein-cell interactions in real-time, will be discussed. Molecular diagnostic methods will also be practically illustrated using proximity ligation assays (PLA) for detection of proteins in solution as well as detection of complex formation in situ.

Protein detection methods like cryogenic electron microscopy (cryo-EM), mass spectrometry technology and its application, chromatography and production of vaccines will be introduced. The development of novel molecular diagnostic methods for different diseases, and their application in medical clinics will be discussed.

Instruction

The instruction is conducted in English through theoretical and practical elements, such as lectures to impart fundamental knowledge, mandatory seminars focused on scientific research methodologies as well as mandatory problem-oriented assignments and presentations that delve into current research fields. Further academic activities include compulsory elements such as laboratory exercises, computer practical sessions and site visits to relevant companies. Literature presentations and mini-symposiums are included to foster discussion and critical analysis.

Assessment

Modes of assessment:

Written examination, written assignment in form of a laboratory report, oral presentation, continuous assessment of compulsory course components

Exceptions to modes of assessment:

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the University's disability coordinator.

Requirements for a passing grade:

To achieve a passing grade for the course, the student requires a passing grade for the written examinations and laboratory report including completed laboratory practicals and related literature assignments as well as oral presentations.

A passing grade requires active participation at the seminars. To pass the requirements of the curriculum, all assigned tasks must be completed.