Cellsortering för enkelcellsgenomik i miljöprov
Tidsperiod: 2013-01-01 till 2017-12-31
Projektledare: Stefan Bertilsson
Medarbetare: Helle Ploug, Jarone Pinhassi, Thijs Ettema, Anders Andersson
Budget: 3 380 000 SEK
Microorganisms drive biogeochemical processes and cycling of most biologically active elements in the biosphere. These ecosystem processes are critical for our long-term existence on earth, yet our knowledge about the diversity, ecology, metabolism and function of these powerful ecosystem engineers is incomplete. New developments in single cell genomics with the ability to perform genome sequencing of uncultured microbial cells are about to revolutionize the way we study the uncultured microbial majority in the biosphere. This methodology will for the first time enable us to rationally describe and explore the evolution, diversity and genetic potential of the so far hidden microbial diversity in nature. Here we apply for funding to obtain an advanced fluorescence activated flow sorter to serve a national and international community of researchers in environmental microbiology, eager to take advantage of these new research opportunities. The specific objectives of the infrastructure are thus to contribute to establishing a first European platform for single cell genomics of microorganisms from the environment in order to (i) resolve the metabolic diversity within natural microbial communities, (ii) enhance metagenomic exploration of ecosystems, (iii) reveal how microbial cells and viruses interact in nature, (iv) uncover the origin of the eukaryotic cell, and (v) enable population genetics analysis of the uncultured.