Studier av den hematopoietiska vävnaden hos sötvattenskräfta som en modell för att förstå evolution av hematopoiesis och adult neurogenes
Tidsperiod: 2015-01-01 till 2018-12-31
Projektledare: Irene Söderhäll
This project will examine how development of the different hemocyte types is regulated at the molecular level, and further examine relationships between the hematopoietic tissue and the neuronal precursor lineage that produces adult-born neurons in the brain of freshwater crayfish. We have discovered a group of cytokines, the astakines, involved in both these processes and will now focus on: 1) finding specific markers for hematopoietic and neurogenic precursor cells 2) how astakine1 affects stem cells to become mature hemocytes via a protein named CHF, and the role of this protein will be studied in detail 3) hematopoiesis is under light control and we have shown an interaction between astakine2 and clock proteins that is induced by melatonin, and we will now study how this interaction affects cell differentiation 4) how the hematopoietic system and adult neurogenesis are coordinately regulated by a serotonin-activated cytokine pathway, by manipulating serotonergic function and the light-dark cycle, we know that serotonin will upregulate astakine1 expression 5) the temporal and spatial interactions of hemocytes and pluripotent stem cells with the neurogenic niche will be defined in real time, using in vitro approaches and time-lapse confocal imaging. The outcome will be increased knowledge about regulation of hematopoiesis, neurogenesis, and evolution of cytokines, and may lead to the characterization of hitherto unknown pathways of importance for developmental biologists.