Den elektroniska 3NANO sensorn för detektering av enstaka DNA-baser
Tidsperiod: 2015-01-01 till 2018-12-31
Projektledare: Shi-Li Zhang
Medarbetare: Klas Hjort, Ralph Scheicher, Zhen Zhang
Budget: 9 000 000 SEK
This project comprises basic interdisciplinary studies in solid state electronics, materials science and materials physics, to create ground-breaking new understanding within one strategically important area of information and communication technology: new tools and technologies for individualised health and care. Genome sequencing has a rapidly increasing use in scientific and clinical applications. Because of the speed and good economy silicon-based electronic genome sequencing can offer, intensive efforts are on-going to improve it with respect to sensitivity, accuracy and signal-to-noise ratio. Our project intends to make a new step in this scientific work. Sequencing an individual DNA strand is expected to lead to lower costs and higher accuracy. Thus, the proposed project aims to explore the fundamental mechanisms of a novel nano-device able to detect single bases on a DNA strand in liquid. It consists of a nanowire transistor embedding a nanobowl for recording base-paring events in electrolytes with, at its bottom, a nanopore functionalised with Phi29 DNA polymerase for a controlled DNA translocation at single base precision. When the device is in the nano-scale, the mechanisms are on a single-event level and existing models based on statistical physics are insufficient to describe them. Our research will, hence, entail a balance between computation modelling based on molecular dynamics simulations and density functional theory with experimental validation.