Diabetes i närbild: Realtidsimaging av immunsvar vid diabetes typ 1
Tidsperiod: 2015-01-01 till 2017-12-31
Projektledare: Gustaf Christoffersson
Budget: 3 150 000 SEK
Why some persons develop type 1 diabetes mellitus is still largely unknown. The incidence of this disease is increasing worldwide, but the reasons for the onset of the autoimmune process killing the insulin-producing beta cells remain hypothetical. Also, the mechanisms of immune cell recruitment and their actions in the pancreas are not completely understood. This proposal is divided into two sub-aims, where the first aims to answer fundamental questions regarding the involvement of innate immune cells in the onset of diabetes, and the second aims to revisit the antigen-specificity requirements for T-cells infiltrating the pancreas during diabetes onset. A unique in vivo imaging method of the mouse pancreas through multiphoton microscopy and whole-body immune cell tracking by magnetic resonance imaging will be used. With new reporter mice with photoinducible fluorescent proteins, correlative microscopy/flow cytometry will increase the information gathered on each immune cell. Findings in the mouse model will be translated to human pathology by using material from donated diabetic human pancreata. The experiments will be performed in the lab of Prof von Herrath at the La Jolla Institute for Allergy and Immunology. The spatiotemporal resolution of the imaging model in this lab, and the powerful infrastructure and research environment, including collaborations in innate immunity and whole-body imaging, of the institute will enable viewing the onset of diabetes from new angles.