Blodkropp eller nervcell - reglering av stamcellsutveckling i kräfta

Tidsperiod: 2019-01-01 till 2022-12-31

Projektledare: Irene Söderhäll

Finansiär: Vetenskapsrådet

Bidragstyp: Projektbidrag

Budget: 2 810 000 SEK

The main focus for this project is to understand hematopoiesis and neurogensis in crustaceans using the freshwater crayfish Pacifastacus leniusculus as a model animal. The hematopoietic tissue of crayfish contains stem cells, which form mature blood cells but also participate in adult neurogenesis. Because of this connection between the circulatory and the nervous system studies of hematopoiesis in crustaceans are of evolutionary interest and will broaden our view about how these processes are diversely regulated in different invertebrate species. In this four-year project we will analyze different stages in the hematopoietic process by a quantitative global proteomics approach, where peptide-maps in the different cell types will be compared and proteins that are likely to be specific for different stages and cell lines leading to blood cells and neuronal precursors will be identified. We will use this information to find marker proteins for different stages in development, and to find out how the production of different blood cells is regulated during an infection. Further, we will study in detail the extracellular matrix proteins in the hematopoietic tissue and their role in regulating cell release. Our research group has a well-established knowledge in the field of invertebrate immunity and this project will give knowledge about how blood cells are produced during an infection, and in addition opens for new insights into how reprogramming of cells is regulated naturally.