Ekotoxikologi-på-ett-chip

Tidsperiod: 2020-01-01 till 2023-12-31

Projektledare: Lars Behrendt

Finansiär: Vetenskapsrådet

Bidragstyp: Bidrag för anställning eller stipendier

Budget: 3 332 000 SEK

Studies in ecotoxicology traditionally focus on exposing organisms to a single stressor, for example, a chemical. But rarely does the resulting data provide the predictive power to understand the outcome of combined stressors – a more likely scenario in natural environments, where pollutants are just one of many complex physico-chemical factors like temperature that can affect organisms in unpredictable ways. To improve our ability to forecast the environmental effects of chemicals, the research in this 4-year proposal will measure the impact of simultaneous environmental and toxicological stressors on cellular physiologies in laboratory-engineered environments, in high throughput. A team consisting of a postdoctoral researcher, a PhD student and the main applicant, will during the first two years of the project, link microfluidic technologies and quantitative phase imaging into an ‘ecotoxicology-on-a-chip’ approach. The resulting methods will, for the remainder of the project duration, be used to systematically expose two model organisms of either ecological (the coral symbiont Symbiodinium) or toxicological relevance (zebrafish) to a vast number of temperature and pollutant combinations. The resulting data and herein developed methods, will significantly advance our experimental abilities to quantify environmentally relevant stress-effects across trophic levels and thereby improve the prediction of chemical risks in aquatic habitats.