Flow Cytometry & Cell Sorting

Flow Cytometry is a fast, versatiles and sensitive technology that allows to analyze up to 20.000 (and even more) particles per second. Particles can be cells or small organisms of 0.3 to 60 micrometers. Single particle suspensions are needed to be ´fed´ into and analyzed by the Flow Cytometer. The particles pass through laser light of different wavelength. Light scatter measurements give insight into properties like (relative) size and internal complexity. Cells can be subjected to different “stainings”, which give insight into the presence or absence of certain proteins (in the cell or the cell´s membrane) and, more importantly the quantity of such proteins (per cell). Other "stainable" parameters are for example DNA, Mitochondria, membranes and more. Cell populations can be identified by such means, and hence results about the status of cells, tissue and organisms can be won.
In order to get hold of such identified populations of cells (or particles), one may utilize a Cell sorter. Sorted and therefore isolated cells (or particles) can now be used for further analysis or experimentations. Biovis Flow Staff can help you analyzation and Cell sorting. We have experience with a wide range of samples and applications.If you have general or specific request or suggestions concerning Flow Cytometry feel free to contact BioVis Staff member Dirk Pacholsky.

In order to use any of our instruments - you need to either book a full service or attend to our Instrument introductions. Application to instrument introduction can be found under Introductions. Price settings are found under Start here menue.

Instruments are bookable over our booking system.

Flow Cytometer

Cyoflex S & LX

Cytoflex S and LX (Beckman Coulter) are easy to use Flow Cytometers with the plate loader capacities and between 14 to 22 fluorescent parameters. The Optical conficgurations for the cytoflex S & LX can be downloaded below under "Information download"

Summary of Cytoflex S (stands @ BMC!)

4 Lasers (405/488/561/633)
14 fluorescent parameters plus FSC/SSC
input tubes: 5ml, 2ml
plate loader: 96 well plate

Summary of Cytoflex LX

5 Lasers (355/405/488/561/633)
22 fluorescent parameters plus FSC/SSC
input tubes: 5ml, 2ml
plate loader: 96 well plate, deep well 96 plate

BioVis gratefully acknowledges the financial support from SciLifLab Uppsala and MedFarm FISK, and IGP department which has allowed the purchase of the Cytoflex LX and S.

Image: Isoline representation of Flow Cytometry Data

Cell Sorter

CYTOFLEX SRT

The Cytoflex SRT (Beckman Coulter)is a cell sorter that can be used independently by experienced and trained customers, otherwise full service applies.

Summary of Cytoflex SRT

4 Lasers (405/488/561/633)
14 fluorescent parameters plus FSC/SSC
input tubes: 5ml
output tubes: 5ml, 15ml and 2ml
output plates: 6,24,48,96,384, deep well 96
sort modi (enrich, purity, single cell)
sorting default or straight down (sorted drop is charged or not charged)
4 way sort (max 4 tubes) and freedom to choose different sort modi to different tubes

BioVis gratefully acknowledges the financial support from SciLifLab Uppsala and MedFarm FISK, which has allowed the purchase of the Cytoflex SRT.

ARIA III

On the AriaIII (Beckton Dickinsson) cell sorting is usually a full service option only - meaning that BioVis staff will do the actual sorting for you. For more information and requests for sorting please contact staff.

Summary of AriaIII

4 Lasers (405/488/561/633)
12 fluorescent parameters plus FSC/SSC
input tubes: 5ml, 15ml
output tubes: 5ml, 15ml and 2ml
output plates: 6,24,48,96,384,
sort modi (enrich, purity, single cell)
sorting default (sorted drop is charged)
4 way sort (max 4 tubes) with same sort modi on all tubes

BioVis gratefully acknowledges the financial support from MedFarm FISK, IGP department and the former Uppsala branch of Ludwig Institute of Cancer Research, which allowed us to the purchase the BD AriaIII.

Image: Stream of Sorter Cytoflex SRT

Information Download Flow Cytometry

Below you find download links concerning more information about the Flow Instruments and Flow Node. All downloads are pdfs

BioVis Manual Pdf, 728 kB.: Cytoflex (S/LX)
BioVis Manual Pdf, 2 MB.: Cytoflex SRT
Optical Configurations Pdf, 107 kB. of Cytoflex S/LX/SRT & AriaIII
You find more ressources under and Course menue