Metabolomik

Metabolomik är som forskningsområde inte lika välutvecklat som till exempel bioanalys avseende metoder och validering. Därför fokuserar vi mycket av vår forskning på att säkerställa hög datakvalitet och att utarbeta nya och förbättrade metoder.

Vi samarbetar även med flera grupper i projekt som bland annat syftar till att förbättra mekanistisk förståelse, prognostik och diagnostik för olika cancerformer.

(the following section only available in English)

Read our review, LC–MS based global metabolite profiling: the necessity of high data quality (Metabolomics (2016) 12:114), for an extended discussion on the topic as well as information on current best practices and suggestions.

Analytical methods for metabolomics

Untargeted metabolomics imply the simultaneous measurement of hundreds to thousands of metabolites. While this might not entail all metabolites in a given sample, it places heavy demands on all stages of the analysis in terms of foremost reproducibility and selectivity.

These demands need to affect the entire study, from experimental design, through sampling, sample preparation, separation, detection, data processing and statistical analysis to biological hypothesis generation.We therefore strive at developing methods better suited to the needs of each study; not only regarding reporducibility and selectivity, but also to meet demands on e.g. sample preparation, separation or detection.

For example, cell quenching and harvesting using nitrogen snap freezing as opposed to methanol washing allows for more tailored sample preparation as described in Metabolic profiling of epithelial ovarian cancer cell lines: evaluation of harvesting protocols for profiling using NMR spectroscopy (Bioanalysis (2015) 7:157).

Applied studies

We collaborate with several research groups within Uppsala University, at Uppsala Academic Hospital, Karolinska Institutet and Queen Mary University of London. Many studies are focused around the mechanistics, prognostics and diagnostics of various forms of cancer; other studies concern neurotoxins or nutrition interventions.

Samples
We routinely analyze biofluids like blood or urine samples, as well as harvested in vitro cell samples, although other samples are handled as well.

Facilities
We use both NMR, UPLC-HRMS and UPLC-MS/MS depending on the needs of each respective study. To that end, we currently employ:

• Bruker Avance 600 MHz with cryoprobe
• Waters Acquity I-class UPLC
• Waters Synapt G2-S Q-TOF
• Waters QuattroMicro Tandem Quadrupole