Widersten Group
![Summary reaction scheme for the reactions catalysed by the enzymes studied by the Widersten group. The crystal structures of the enzymes are inserted in the image.](/images/200.2293423318ecba401bd20578/1713273759971/Widersten_startsida_1932px.jpg)
Enzymology and directed protein evolution
Enzyme catalysts are characterized by an unprecedented specificity for reactant molecules that will be acted upon. Often only a specific stereoisomer of a chiral molecule will be efficiently converted by a particular enzyme. Such specificity is of high value if applied to synthesis of chiral organic compounds where product purity is essential.
We study structure/function relationships in enzymes and use what we learn for the development of new and useful biocatalysts for specific and efficient catalysis of reactions generating chiral and pro-chiral product molecules.
Publications
Part of FEBS Open Bio, p. 655-674, 2024
- DOI for Structural enzymology studies with the substrate3S-hydroxybutanoyl-CoA: bifunctional MFE1 is a less efficient dehydrogenase than monofunctional HAD
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Part of Faraday discussions, 2024
- DOI for High-throughput selection of (new) enzymes: phage display-mediated isolation of alkyl halide hydrolases from a library of active-site mutated epoxide hydrolases
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Part of The FEBS Journal, p. 465-481, 2023
- DOI for Structures of lactaldehyde reductase, FucO, link enzyme activity to hydrogen bond networks and conformational dynamics
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Part of Catalysis Science & Technology, 2023
- DOI for Engineered Aldolases Catalyzing Stereoselective Aldol Reactions Between Aryl-Substituted Ketones and Aldehydes
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Part of IUCrJ, p. 437-447, 2023
- DOI for Crystal structures and kinetic studies of a laboratory evolved aldehyde reductase explain the dramatic shift of its new substrate specificity
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Facile Synthesis of 2-Hydroxyacetophenone from Racemic Styrene Oxide Catalyzed by Engineered Enzymes
Part of Biotechnology letters, p. 985-990, 2022
- DOI for Facile Synthesis of 2-Hydroxyacetophenone from Racemic Styrene Oxide Catalyzed by Engineered Enzymes
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Structure and Mechanism of a Cold-Adapted Bacterial Lipase
Part of Biochemistry, p. 933-942, 2022
- DOI for Structure and Mechanism of a Cold-Adapted Bacterial Lipase
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Part of ACS Catalysis, p. 9115-9128, 2020
- DOI for The Role of Substrate-Coenzyme Crosstalk in Determining Turnover Rates in Rhodococcus ruber Alcohol Dehydrogenase
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Engineering aldolases for asymmetric synthesis
Part of Methods in Enzymology, p. 149-167, 2020
Part of Journal of Organic Chemistry, p. 6982-6991, 2019
Part of Synlett, p. 1187-1190, 2018
Part of Biochemical Journal, p. 2395-2416, 2018
Part of Biochemistry, p. 5877-5885, 2018
Epoxide Hydrolysis as a Model System for Understanding Flux Through a Branched Reaction Scheme
Part of IUCrJ, p. 269-282, 2018
- DOI for Epoxide Hydrolysis as a Model System for Understanding Flux Through a Branched Reaction Scheme
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Part of ACS Catalysis, p. 7526-7538, 2018
- DOI for Stereo- and Regioselectivity in Catalyzed Transformation of a 1,2-Disubstituted Vicinal Diol and the Corresponding Diketone by Wild Type and Laboratory Evolved Alcohol Dehydrogenases
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Part of Biochemistry, p. 1059-1062, 2018
Part of The FEBS Journal, p. 3895-3914, 2017
- DOI for Relaxation of Nonproductive Binding and Increased Rate of Coenzyme Release in an Alcohol Dehydrogenase Increases Turnover With a Non-Preferred Alcohol Enantiomer
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Conformational Diversity and Enantioconvergence in Potato Epoxide Hydrolase 1
Part of Organic and biomolecular chemistry, p. 5639-5651, 2016
- DOI for Conformational Diversity and Enantioconvergence in Potato Epoxide Hydrolase 1
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Part of Chemical Science, p. 1415-1421, 2016
- DOI for Linking coupled motions and entropic effects to the catalytic activity of 2-deoxyribose-5-phosphate aldolase (DERA)
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Part of ChemBioChem (Print), p. 1693-1697, 2016
- DOI for Laboratory evolved enzymes provide snapshots of the development of enantioconvergence in enzyme-catalyzed epoxide hydrolysis
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Directed evolution on FucO - structural explanations for changes in substrate scope
Part of Protein Science, p. 199-200, 2015
Part of ChemBioChem (Print), p. 2595-2598, 2015
Expanding the catalytic triad in epoxide hydrolases and related enzymes
Part of ACS Catalysis, p. 5702-5713, 2015
- DOI for Expanding the catalytic triad in epoxide hydrolases and related enzymes
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Design of a PDZbody, a bivalent binder of the E6 protein from human papillomavirus
Part of Scientific Reports, 2015
- DOI for Design of a PDZbody, a bivalent binder of the E6 protein from human papillomavirus
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Protein engineering for development of new hydrolytic biocatalysts
Part of Current opinion in chemical biology, p. 42-47, 2014
- DOI for Protein engineering for development of new hydrolytic biocatalysts
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Kinetic characterization of Rhodococcus ruber DSM 44541 alcohol dehydrogenase A
Part of Journal of Molecular Catalysis B, p. 68-78, 2014
Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution
Part of The FEBS Journal, p. 2387-2398, 2014
Part of ACS Catalysis, p. 3016-3025, 2013
Part of Biochemistry, p. 7627-7637, 2012
Part of Biochimica et Biophysica Acta - Proteins and Proteomics, p. 561-570, 2012
One-step enzyme extraction and immobilization for biocatalysis applications
Part of Biotechnology Journal, p. 463-469, 2011
Part of Journal of Molecular Catalysis B, p. 148-155, 2010
Part of ChemBioChem (Print), p. 1422-1429, 2010
Part of Biochemistry, p. 2297-2304, 2010
Part of Archives of Biochemistry and Biophysics, p. 165-173, 2010
Deep eutectic solvents (DESs) are viable cosolvents for enzyme-catalyzed epoxide hydrolysis
Part of Journal of Biotechnology, p. 169-171, 2010
Structure-function relationships of epoxide hydrolases and their potential use in biocatalysis
Part of Biochimica et Biophysica Acta, p. 316-326, 2010
Part of Protein Science, p. 1275-1284, 2008
- DOI for Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability
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Substrate-dependent hysteretic behavior in StEH1-catalyzed hydrolysis of styrene oxide derivatives
Part of The FEBS Journal, p. 6309-6320, 2008
Part of Biochemistry, p. 2466-2479, 2007
Part of Biochemistry, p. 205-212, 2006
X-ray structure of potato epoxide hydrolase sheds light on its substrate specificity
Part of Protein Science, p. 1628-1637, 2006
Catalysis of potato epoxide hydrolase, StEH1
Part of Biochemical Journal, p. 633-640, 2005
Part of Biochimica et Biophysica Acta - Proteins and Proteomics, p. 213-221, 2005
Part of Protein Engineering Design & Selection, p. 537-546, 2005
Part of Biochemical Journal, 2004
Part of Biochemistry, p. 12038-12047, 2004
Part of Int J Cancer, p. 700-705, 2002
Yeast glyoxalase I is a monomeric enzyme with two active sites
Part of Journal of Biological Chemistry, p. 1845-1849, 2001
Chlorine kinetic isotope effects on the haloalkane dehalogenase reaction
Part of Journal of the American Chemical Society, p. 4550-4555, 2001